Figure 3.

IGF-1/Akt pathway inhibits the RA response through enhancing RARA phosphorylation. (A, B) RA signaling assessment. BMDCs cultured from RARE-lacZ transgenic mice were treated with DMSO, Tamibarotene (4mM) alone or with IGF-1 (500ng/ml).DDAO signaling were measured by flow cytometry after BMDC treated with substrate DDAOG. (C, D) BMDCs were cultured from normal SPF mice, and treated with DMSO, Tamibarotene, IGF-1 and Akt inhibitor (MK2206), then p-Akt, Akt, p-RARα, RARα expression were detected by western blotting. Data are from two or three independent experiments. Graph shows mean and SEM. Data were analyzed by one-way ANOVA. **p < 0.01, ***p < 0.001.