Figure 4.

Retinoic acid receptor alpha regulates type I interferons and stat1 expression. (A–E) BMDCs cultured from RARα^fl/fl and RARα^ΔDC mice, were treated with DMSO, Tamibarotene (4mM) alone or with IGF-1 (500ng/ml), then mRNA expression of IFN-α, IFN-β, and ISGs (IFIT1, IFIT2, MX1) were determined by RT-PCR. (F) ChIP-qPCR for RARα at IFN-α, and IFN-β regulatory element in BMDCs cultured from SPF mice. (G) BMDCs from SPF mice were treated with different concentration of tamibarotene (respectively 0uM, 5uM, 10uM), and stat1 protein expression were measured by western blotting. (H) stat1 expression in BMDCs from RARα^fl/fl and RARα^ΔDC mice were detected by immunofluorescence method. Data are from at least two independent experiments. Graph shows mean and SEM. Data were analyzed by one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. ns, not significant.