FIG. 3.

PCR analysis for the detection of the ribC DNA region in B. henselae and in other Bartonella species. PCR with primers PBH3 and PBH4 (Fig. 1) were used to amplify a 1.7-kb DNA fragment which carries the ribC gene (arrow). PCR analysis was performed with 100 ng (lane 1), 10 ng (lane 2), and 1 ng (lane 3) of isolated total DNA from B. henselae FR96/K4 as the target. Total DNA from B. henselae Houston-1 (100 ng) served as a positive control (lane 4). PCR analysis with primers PBH3 and PBH4 generated products with sizes similar to those of DNA of other B. henselae isolates, B. quintana, B. bacilliformis, and B. clarridgeiae, listed in Table 1 (not shown). Lanes M, marker DNA fragments. The PCR products were separated on a 1.2% agarose gel and stained with ethidium bromide.