FIG. 5.

Species-specific differentiation of Bartonella species by PCR analysis with primers designed from the ribC DNA region. Primers designed from the ribC DNA regions of B. bacilliformis (PBH-L1 and PBB-R1), B. clarridgeiae (PBH5 and PBH15), B. henselae (PBH-L1 and PBH-R1), and B. quintana (PBH-L1 and PBQ-R1) (as indicated at the top) were used for PCR analysis of Bartonella species under stringent species-specific conditions (Table 2). DNA isolated (100 ng) from B. bacilliformis (lanes 1), B. clarridgeiae (lanes 2), B. henselae (lanes 3), and B. quintana (lanes 4) was analyzed. Sizes of PCR products are listed in Table 2. Lanes M, marker DNA fragments. The PCR products were separated on a 1.6% agarose gel and stained with ethidium bromide.