TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| B. bacilliformis ATCC 35685 | American Type Culture Collection | |
| B. clarridgeiae | ||
| 73 | From cat | 19 |
| R13762 | From cat | 29 |
| R8051 | From cat | 29 |
| B. henselae | ||
| Houston-1 | Variant IV, from HIV patient | 36 |
| FR96 strains | Variants I to IV, From cat | 42 |
| R8015 | From cat | E. Marston (29) |
| R8016 | From cat | E. Marston (29) |
| R8017 | From cat | E. Marston (29) |
| R8018 | From cat | E. Marston (29) |
| B. quintana | ||
| CIP103739 | CIP | |
| München | A. Sander (44) | |
| E. coli | ||
| BSV23 | thi hsd (r− m−) ribC::Tn5 Km | A. Bacher |
| RR28 | thi leu pro lac gal ara xyl endA recA hsd (r− m−) pheS12 supE44 | 38 |
| Rib2 | RR28 with a point mutation in ribD | A. Bacher |
| TOP10 | F−mcrAΔ(mrr-hsdRMS-mcrBC) f80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen |
| Plasmids | ||
| pZERO-2 | Cloning vector; ccdB neo oriColEI Km | Invitrogen |
| pBH-RIBC1 | pZERO-2 with a 2.3-kb PstI DNA fragment carrying the ribC gene from B. henselae; Km | This study |
a
Km, resistant to kanamycin.