TABLE 2.
Primers and conditions used for the differentiation of Bartonella species by PCR
| Species is specific for PCR assay | Primers | Nucleotide sequence | Annealing temp (°C) | PCR product size (bp)a |
|---|---|---|---|---|
| B. clarridgeiae | PBC5 | TACATAACGAGCCAATT | ||
| PBC15 | TAGCTTTAGAACAATATGGT | 50 | 895 | |
| B. henselae | PBH-L1 | GATATCGGTTGTGTTGAAGA | ||
| PBH-R1 | AATAAAAGGTATAAAACGCT | 55 | 393 | |
| B. bacilliformis | PBH-L1 | Same as for B. henselae | ||
| PBB-R1b | AAAGGCGCTAACTGTTC | 62 | 386 | |
| B. quintana | PBH-L1 | Same as for B. henselae | ||
| PBQ-R1b | AAAGGGCGTGAATTTTG | 60 | 390 | |
| Various species | PBH3 | CCAAGTGCTACATAACCATC | ||
| PBH4 | CGGGTTGTTATTGCTCTTAC | 55 | 1,723b |
a
The sizes of the PCR products were calculated from the riboflavin synthesis gene cluster of each species.
b
The size of a fragment from B. henselae was calculated.