Table 1.
Primers used for gene cloning and RT-qPCR analysis.
| Gene | Primer sequences (5′→3′) | Product size (bp) | Purpose |
|---|---|---|---|
| caIFITM1 | Fa : ACATCCGCAGTGACACG | 200 | RT-qPCR for detection of caIFITM1 |
| Rb:CACGACCAAGGCCGAG | |||
| caIFITM2a | F:AACGTTCCGGTGGAGA | 217 | RT-qPCR for detection of caIFITM2a |
| R:TGGTCAGGAGGAGGC | |||
| caIFITM2b | F:GTCCCGGAGGAGACG | 210 | RT-qPCR for detection of caIFITM2b |
| R:GGATATGATGCCCAG | |||
| caIFITM3 | F:CCCCACCTACGAGATGC | 93 | RT-qPCR for detection of caIFITM3 |
| R:ATCACGGTGGTAATCG | |||
| caIFITM5 | F:CCAAAGCCAAGTGCTAC | 159 | RT-qPCR for detection of caIFITM5 |
| R:AGTCATAGTCCGAGTCCT | |||
| Mx1 | F:TGATATGCTGCACACGATAAC | 181 | RT-qPCR for detection of Mx1 |
| R:GATCTGCTCCATTTGGAAGTG | |||
| CIV M | F:TGATCCTCTCGTTATTGCCGCAAG | 159 | RT-qPCR for detection of CIV |
| R:CACTCTGCTGTTCCTGCCGATAC | |||
| IFN-α | F:AACACGTCCTCTGCTCCTTG | 153 | RT-qPCRfor detection of IFN-α |
| R:GTAGGTCCTCAGGGTGGAGT | |||
| GAPDH | F:CATCACCATCTTCCAGGAGCG | 146 | RT-qPCR for detection of GAPDH |
| R:AGATGATGACCCTTTTGGCT | |||
| caIFITM1 | F:ACACACAAACATCCCCA | 479 | Amplification of caIFITM1 |
| R:CGGAGCAGAGGGCTGGG | |||
| caIFITM2a | F:CCTGTGCCTCCCCTCGAGA | 480 | Amplification of caIFITM2a |
| R:GTCTGTGCACCCGGAGCAG | |||
| caIFITM2b | F:TGGCCACCTGCACTCTT | 441 | Amplification of caIFITM2b |
| R:GGGGCCCAGGGCGTCCT | |||
| caIFITM3 | F:GCACCTGCCACCATGAGCC | 472 | Amplification of caIFITM3 |
| R:AGGCCTCCGGCGCCGACTA | |||
| caIFITM5 | F:ATGGACACGGCGTACCCCCGC | 527 | Amplification of caIFITM5 |
| R:CTGGAGTCAGGGTTCCGGGCA |
aForward; bReverse.