Table 1.
Baseline Patient Characteristics
| Flow Cytometric Analysis |
RNA-seq |
CyTOF |
||||
|---|---|---|---|---|---|---|
| CD Patients (n = 27) | HC Subjects (n = 7) | CD Patients (n = 4) | HC Subjects (n = 3) | CD Patients (n = 3) | HC Subjects (n = 2) | |
| Sex | ||||||
| Female | 16 (76.2) | 4 (57.1) | 3 (75) | 2 (66.7) | 2 (66.7) | 1 (50) |
| Male | 5 (23.8) | 3 (42.8) | 1 (25) | 1 (33.3) | 1 (33.3) | 1 (50) |
| Age, y | 24 (20-32) | 50 (46–60) | 49 (30–54) | 36 | 46 | 36 |
| Smoking status | ||||||
| Yes | 11 (52.4) | 0 (0) | 2 (50) | 2 (66.7) | 2 (66.7) | 2 (100) |
| No | 7 (33.3) | 7 (100) | 0 (0) | 1 (33.3) | 0 (0) | 0 (0) |
| Ceased | 3 (14.3) | 0 (0) | 2 (50) | 0 (0) | 1 (33.3) | 0 (0) |
| Duration of complaints before ileocolonoscopy, wk | 14 (9–23) | NA | 4 (1–6) | NA | 4 | NA |
| Calprotectin, μg/g | 231 (156–487) | NA | 120 (51–728) | NA | 139 | NA |
| CRP, mg/L | 25 (11–62) | NA | 9 (4–19) | NA | 4 | NA |
| Treatment at ileocolonoscopy | ||||||
| None | 22 (81.4) | 7 (100) | 4 (100) | 3 (100) | 3 (100) | 2 (100) |
| Mesalamine | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| Steroids | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| Thiopurine | 1 (4.5) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| Mesalamine + thiopurine | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| Anti-TNF | 3 (13.6) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| Anti-IL12/23 | 1 (4.5) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| HBI score | ||||||
| <5, remission | 2 (7.4) | NA | 1 (25) | NA | 0 (0) | NA |
| 5–7 mild disease | 12 (44.4) | 2 (50) | 2 (66.7) | |||
| 8–16 moderate disease | 9 (33.3) | 1 (25) | 1 (33.3) | |||
| >16 severe disease | 4 (14.8) | 0 (0) | 0 (0) | |||
| SES-CD score | ||||||
| 0–2 inactive disease | 0 (0) | NA | 0 (0) | NA | 0 (0) | NA |
| 3–6 mild disease | 8 (29.6) | 1 (25) | 1 (33.3) | |||
| 7–15 moderate disease | 12 (44.4) | 2 (50) | 1 (33.3) | |||
| ≥16 severe disease | 7 (25.9) | 1 (25) | 3 (33.3) | |||
| Montreal CD | ||||||
| Location | 10 (37) | 3 (75) | 2 (66.7) | |||
| L1: ileal | 0 (0) | NA | 0 (0) | NA | 0 (0) | NA |
| L2: colonic | 17 (73) | 1 (25) | 1 (33.3) | |||
| L3: ileocolonic | ||||||
| Behavior | 23 (85.2) | 1 (25) | 1 (33.3) | |||
| B1: nonstricturing, nonpenetrating | ||||||
| B2: stricturing | 3 (11.1) | 3 (75) | 2 (66.7) | |||
| B3: penetrating | 1 (3.7) | 0 (0) | ||||
Values expressed in n (%) or as median with interquartile range.
CD, Crohn’s disease; CRP, C-reactive protein; HBI, Harvey-Bradshaw index; HC, healthy control; RNA-seq, RNA-sequencing; SES-CD, simple endoscopic score for CD; TNF, tumor necrosis factor.