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. 2021 Oct 14;12:750841. doi: 10.3389/fimmu.2021.750841

Figure 5.

Figure 5

Reduced IL-1β is not responsible for the protective effect against colitis in Gsdmd-deficient mice. (A) ELISA analysis of IL-1β protein levels in colon homogenates from WT or Gsdmd-/- mice on Day 0 or Day 8 of the colitis model. (B) Gsdmd-/- mice simultaneously received a daily injection of 0.5 μg IL-1β, 1 μg IL-1β or PBS, while WT mice were injected with PBS (n = 5/group). The body weight change of the above mice was monitored during the progression of DSS-induced colitis. (C) Bleeding score and stool score of WT or Gsdmd-/- mice on Day 6 of the colitis model as in (B). (D, E) Colon length (D) and H&E histology (E) of WT or Gsdmd-/- mouse colons on Day 8 of the colitis model as in (B). Data are representative of two (A–E) independent experiments (mean ± SEM in A–D). *p < 0.05, **p < 0.01 by Student’s t test.