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. 2021 Oct 14;12:729308. doi: 10.3389/fmicb.2021.729308

FIGURE 1.

FIGURE 1

Deletion of S protein alters properties of surface chemistry in GBS. (A) Photo demonstrating sedimentation phenotype of WT, S protein deletion, and complemented GBS strains. (B) Hydrophobicity assay of WT, S protein deletion, and complemented GBS strains. Significance was determined using Tukey’s Multiple Comparisons Test (∗∗p < 0.01). (C) Transmission electron microscopy of WT, S protein deletion, and complemented GBS strains. Images to the right are representatives of images on the left, blown up to demonstrate cell wall and capsule morphology. (D) Capsule thickness quantified in ImageJ. Significance was determined using Tukey’s Multiple Comparisons Test (∗∗p < 0.01; ∗∗∗p < 0.001; *⁣*⁣**p < 0.0001). (E) Capsule detected in supernatant using Stains-all and normalized against a Streptococcus equi capsule standard (∗p < 0.05).