FIGURE 6.

Proteome-based evaluation of S protein-dependent host responses. (A) Schematic of mouse organ proteome experiment. (B) Venn diagram demonstrating overlap in proteins detected and quantified in blood and spleens following proteome analysis. (C) Scatterplot demonstrating Log₂ fold change of WT infected vs. uninfected GBS strains for blood and spleen proteome data. Highlighted datapoints represent significantly altered proteins (π score > 1.5). (D) Scatterplot demonstrating Log₂ fold change of S protein deletion mutant infected vs. uninfected GBS strains for blood and spleen proteome data. Highlighted datapoints represent significantly altered proteins (π score > 1.5). (E) Venn diagram demonstrating significantly altered proteins in spleen comparison of WT or S protein deletion mutant strain compared to uninfected. (F) Protein interaction network for the union of significantly altered spleen proteins in comparison of WT or S protein deletion mutant strain vs. uninfected. (G) Heatmap demonstrating proteasome components upregulated in spleens of animals infected with S protein deletion mutant compared to WT GBS infected or uninfected spleens. (H) Volcano plot demonstrating significantly altered (p < 0.01) proteins in the comparison of brains collected from WT vs. S protein deletion mutant GBS following normalization against uninfected. (I) Non-redundant top enriched functional terms from proteins significantly increased in brains of mice infected with WT GBS compared to the S protein deletion mutant. (J) Non-redundant top enriched functional terms from proteins significantly increased in brains of mice infected with S protein deletion mutant compared to the WT strain. (K) Mean relative abundance heatmap of clathrin-related proteins significantly upregulated in WT GBS infected brains compared to S protein deletion mutant infected brains. (L) Mean relative abundance heatmap of Insulin-like Growth Factor regulation-related proteins significantly upregulated in WT GBS infected brains compared to S protein deletion mutant infected brains.