Fig. 4.

Inactivation of mTOR suppresses mRNA levels both HMMR and HAS in dose dependent manner. (A, B) Total RNA was isolated from PC-3 (A) or LNCaP (B) cells stimulated with mTOR inhibitors (KU; KU0063794, Rapa; Rapamycin, TR2; Torin2) for 24 h. (C, D) Cells were exposed to various doses of Torin2 for 24 h. Quantitative real time PCR analysis was performed to determine message levels of HMMR and HAS isoforms. Data shown are the mean ± SD of triplicate determinations and representative of two or three independent experiments (A-D) (^#p<0.05 vs. control, *p<0.05, **p<0.01, ****p<0.0001 vs. control).