Skip to main content
. 2021 Oct 14;9:723801. doi: 10.3389/fcell.2021.723801

FIGURE 1.

FIGURE 1

Radiation induced cell death in MDA-MB-231 breast cancer cells. MDA-MB-231 were pretreated with different inhibitors of cell death, i.e., 3MA (2 mM), spautin-1 (3 μM), rapamycin (5 nM), z-VAD-fmk (10 μM), Necrostain-1 (10 μM), Digitoxigenin (Di, 5 μM) and cell deaths were quantified at 24, 48, 72 h after radiation (A–C). Cells were pretreated with FeCl3 (30 μM, pretreated for 3 h), DFO (0.1 mM), Ferrostain-1 (5 μm) for 1 h, cell death was quantified at 48 h after 8 Gy radiation (D). Electron microscopy analysis of autophagosome in the MDA-MB-231 cells was made after radiation treatment. Red arrow pointed to autophagosomes (E). These results were representative of three independent experiments and expressed as means ± SD. P-values were calculated using repeated measure ANOVA with P < 0.05 being considered as statistical significance, *P < 0.05, **P < 0.01, and ***P < 0.001.