FIGURE 3.

Radiation induced Lysosomal membrane permeabilization (LMP) in MDA-MB-231 breast cancer cells. MDA-MB-231 cells were treated with 4 Gy, 8 Gy radiation respectively, the amount of LMP was detected at 4, 8 h with LysoTracker and analyzed by flow cytometry (A–C) or with AO staining (D). In the presence or absence of NH₄Cl, the Ferritin (FTH) expression were detected after 8 Gy treatment at 24 h (E). Cells were pretreated with 3MA (2 mM) or MG132 (1 μM) for 1 h before 8 Gy radiation, the Ferritin (FTH) expression were detected at 24 h (F). Cells were pretreated with 3MA before 8 Gy radiation, the intracellular iron was evaluated with Prussian blue staining at 24 h by light microscopy (G). These results were representative of three independent experiments and expressed as means ± SD. P-values were calculated using repeated measure ANOVA with P < 0.05 being considered as statistical significance, *P < 0.05.