| Reagents | Volume |
|---|---|
| Linearized ipUSEPR plasmid (60–100 ng/μL) | 1.5 μL |
| 10X T4 ligase buffer (supplied with T4 DNA ligase) | 0.6 μL |
| T4 DNA ligase (NEB # M0202S) | 0.3 μL |
| Master Mix (all above three products) | 2.4 μL |
| 1/2000 diluted oligo (remember adding H2O control) | 3.6 μL |
| Total | 6 μL |
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