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Growth media for Lenti-X 293T cell line
| Reagent | Final concentration |
|---|---|
| DMEM | N/A |
| FBS | 10% (v/v) |
| Penicillin-Streptomycin (100X) | 1% (v/v) |
| Sodium pyruvate (100 mM) | 1% (v/v) |
| MEM non-essential amino acid (100X) | 1% (v/v) |
| GlutaMAX (100X) | 1% (v/v) |
Note: Mix all these components and filter the media using sterile disposable vacuum filter with PES membrane. This is complete DMEM media. Store the media at 4°C for up to 1 month.
