Growth media for K-562 cell line

Reagent	Final concentration
RPMI-1640	N/A
FBS	10% (v/v)
Penicillin-Streptomycin (100X)	1% (v/v)
Sodium pyruvate (100 mM)	1% (v/v)
Minimum Essential Media (MEM) non-essential amino acid (100X)	1% (v/v)
GlutaMAX (100X)	1% (v/v)
2-Mercaptoethanol (50 mM)	0.01% (v/v)

Note: K-562 cell line is grown in complete RPMI media described above. Mix all above components and filter the media using sterile disposable vacuum filter with PES membrane. Store the media at 4°C for up to 1 month.