Single guide RNA (sg) target sequences
| Name | Forward sequence (5’→3′) | Reverse sequence (5’→3′) | Application |
|---|---|---|---|
| Control (Scramble) sgRNA | GTAGCGAACGTGTCCGGCGT | ACGCCGGACACGTTCGCTAC | CRISPRko/i/a |
| RFP sgRNA | GTCACCACATACGAAGACGG | CCGTCTTCGTATGTGGTGAC | CRISPRko |
| IL2RG sgRNA (1) | GGTGCAGTACCGGACTGACT | AGTCAGTCCGGTACTGCACC | CRISPRko |
| IL2RG sgRNA (2) | AGCCGCTTTAACCCACTCTG | CAGAGTGGGTTAAAGCGGCT | CRISPRko |
| IL2RG sgRNA (1) | TGGTAATGATGGCTTCAACA | TGTTGAAGCCATCATTACCA | CRISPRi |
| IL2RG sgRNA (2) | AGGGATGTGAATGGTAATGA | TCATTACCATTCACATCCCT | CRISPRi |
| IL-15 sgRNA | ACACCTCCCGCGGAGACTGG | CCAGTCTCCGCGGGAGGTGT | CRISPRa |
Forward sgRNAs each with a 5′ overhang “CACCG” and reverse sgRNAs each with 5’ “AAAC” and 3’ “C” overhangs were ordered form integrated DNA technologies (IDT). Refer to the section titled “oligo design for sgRNA production” for sgRNA design details.