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. 2021 Oct 14;12:729851. doi: 10.3389/fimmu.2021.729851

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Copyright © 2021 Wirchnianski, Wec, Nyakatura, Herbert, Slough, Kuehne, Mittler, Jangra, Teruya, Dye, Lai and Chandran

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antibody-antigen binding profiles of NPC2- and IGF2-tagged bsAbs. Kinetic binding curves for NPC2- and IGF2-tagged bsAb:antigen interactions were determined by BLI. (A) 548~NPC2 and (C) 548~IGF2 were loaded onto probes and dipped into analyte solutions containing the indicated concentrations of NPC1-C. (B) MR72~NPC2 and (D) MR72~IGF2 were loaded onto probes and dipped into the indicated concentrations of analyte solutions containing EBOV GP_CL. Grey lines indicate curve fits to a 1:1 binding model. See Table 1 for kinetic binding constants.