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. 2021 Oct 14;12:729851. doi: 10.3389/fimmu.2021.729851

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Copyright © 2021 Wirchnianski, Wec, Nyakatura, Herbert, Slough, Kuehne, Mittler, Jangra, Teruya, Dye, Lai and Chandran

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cellular internalization of Trojan horse bsAbs. (A) Schematic of pHrodo Red-labeled antibody internalization experiment. (B, C) Labeled antibodies were incubated with cells at either 4°C to prevent internalization or 37°C to allow internalization prior to analysis by flow cytometry. Data points represent the percentage of pHrodo Red positive cells; bars represent means ± SD from 3 independent experiments. Group means (bars) were compared using one-way ANOVA with Turkey’s multiple comparisons test. (****P < 0.0001; **P < 0.01; ns, not significant).