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. 2021 Oct 14;12:747140. doi: 10.3389/fmicb.2021.747140

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Copyright © 2021 Chen, Huang, Zhou, Tan, Tan and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic of the gold nanoparticle-based LFB for N. gonorrhoeae-MCDA product visualization. (A) MCDA products (0.5 μl) and running buffer (100 μl) were deposited on the sample pad. (B) The running buffer containing MCDA products moves along the LFB due to capillary action, while streptavidin dye-coated gold nanoparticles (streptavidin-DPNs) are rehydrated in the conjugate region. (C) For positive samples, FAM/biotin-labeled orf1-MCDA amplicons are captured by anti-carboxyfluorescein (FAM) at the test line (TL), and streptavidin-DPNs are captured by biotin-BSA at the control line (CL). For negative outcomes, only streptavidin-DPNs are captured by biotinylated bovine serum albumin (biotin-BSA) at the CL. (D) Interpretation of N. gonorrhoeae-MCDA-LFB assay results; positive – CL and TL appear on the LFB, and negative – only the CL appears on the LFB.