TABLE 1.

Neisseria gonorrhoeae-multiple cross displacement amplification (MCDA) primers used in this study.

Primer name	Sequence and modifications	Length	Gene
F1	5′-ACGTCCACCAATCCATTTGG-3′	20 nt	orf1
F2	5′-GATGGAAGCGGAACGGTT-3′	18 nt
CP1	5′-TGTAGTAGAGCGCGGTATCGGAACGGTCAAAACCTGTTCGC-3′	41 mer
CP2	5′-ACCAACTCCTACAAACGCCTCGTTGGCGGAATAGGCCAATT-3′	41 mer
C1	5′-TGTAGTAGAGCGCGGTATCGGA-3′	22 nt
C1*	5′-Biotin-TGTAGTAGAGCGCGGTATCGGA-3′	22 nt
C2	5′-ACCAACTCCTACAAACGCCTCG-3′	22 nt
D1	5′-AAACCGGCATAGCCGTCG-3′	18 nt
D1*	5′-FAM-AAACCGGCATAGCCGTCG-3′	18 nt
D2	5′-ACTTTGAAGCACCGACC-3′	17 nt
R1	5′-GCTTTGGCGTGTTTGAT-3′	17 nt
R2	5′-TGAACGCGATTACCAAT-3′	17 nt

C1*, 5′-labeled with biotin when used for MCDA-LFB detection; D1*, 5′-labeled with FAM when used for MCDA-LFB detection. FAM, 6-carboxy-fluorescein; nt, nucleotide; mer, monomeric unit.