Figure 3.

Overview of the protocol generating hiPSC-derived astrocytes from NPCs

This timeline recapitulates the main steps to generate astrocytes from hiPSC-derived NPCs with representative pictures of the expected cellular morphology at each intermediate: thawed NPCs, differentiating cells and mature astrocytes. Cells can be banked at the end of the protocol after astrocyte maturation. On the central panel, white arrows indicate NPC niches, blue arrows indicate differentiating neurons and green arrows indicate emerging astrocytes. In the timeline, lozenges approximately represent the frequency of enzymatic passages (see steps 13 to 15 for the procedure). Scale bar: 200 μm.