Table 3.

Seeding density and suggested cell culture surface

Stage	Seeding density	Cell culture surface
NPCs (expansion stage)	50′000 cells/cm2	From 1×T25 (25cm2) at P1 to 4×T150 at P8 (600cm2)
Astrocyte induction stage, D0 to D14	50′000 cells/cm2	2×T150 at D14 (300 cm2)
Astrocyte maturation stage, D14 to 28	40′000 cells/cm2	Up to 6-8×T150 at D28 (900–1200cm2)
Astrocyte maturation stage, D29 to 42	20′000–30′000 cells/cm2	Up to 12-16×T150 at D42 (1800–2400cm2)