Astrocyte induction medium
| Reagent | Final concentration | Amount | ||
|---|---|---|---|---|
| DMEM/F-12 + Glutamax | 485mL | |||
| N2 supplement | 1× | 5mL | ||
| B27 supplement without vitamin A | 1× | 10mL | ||
| EGF | 10 ng/mL | 5μg | ||
| LIF | 10 ng/mL | 5μg | ||
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