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. 2021 Oct 14;12:738047. doi: 10.3389/fmicb.2021.738047

Figure 2.

Figure 2

Cytotoxicity by S. gordonii is mediated through apoptosis in PDL cells. S. gordonii was cultured to mid-log phase in THY media at 37°C. (A) PDL cells were treated with S. gordonii at MOI 1:1,000 for 30, 60, or 180min. The cells were stained with annexin V and PI and then analyzed using flow cytometry. Numbers indicate the percentage of cells in each panel. (B) PDL cells were pre-treated with Z-VAD-FMK (0, 5, or 50μM) for 1h, followed by treatment with S. gordonii at MOI 1:100 for 3h. Trypan blue assay was used to determine the number of viable PDL cells. The cells were counted. One of three similar results is shown. *p<0.05.