Figure 3.

S. gordonii induces reactive oxygen species (ROS) production regardless of its cytotoxic effects in PDL cells. (A) PDL cells were treated with 10μM of DCF-DA for 30min at 37°C. After washing with PBS, the DCF-DA-labeled cells were treated with S. gordonii at MOI 1:10, 1:100, or 1:1,000 for 3h. Fluorescent intensity was analyzed by flow cytometry. (B) PDL cells were pre-treated with 100μM of resveratrol for 1h, followed by treatment with S. gordonii at MOI 1:100 or 1:1,000 for 3h. (C) PDL cells were pre-treated with 10mM NAC for 1h, followed by treatment with S. gordonii at MOI 1:100 for 3h. Trypan blue assay was used to determine the number of viable cells. One of three similar results is shown. ^*p<0.05.