FIGURE 3.

DHT inhibits ASC oligomerization. (A) Western blot analysis of proteins in lysates. from BMDMs with 4 h-LPS priming and then treated with or without different doses of DHT (2.5, 5, 10 μM) for 1 h, or BMDMs treated with varying doses of DHT (2.5, 5, 10 μM) for 1 h and then stimulated with LPS for 4 h. Lamin B was used as an internal control. (B) Production of TNF-α in Sup. from BMDMs as described in (A). (C, D) BMDMs were pretreated with LPS and stimulated with nigericin, ATP, transfected LPS, SiO₂, poly (I: C), or Pam3CSK4-primed BMDMs stimulated with LPS transfection after exposure to DHT. Western blotting analysis of ASC oligomerization in cell lysates.