TABLE 1.
Primer sequences used for qRT-PCR analysis of defense-related genes (Trotel-Aziz et al., 2019).
| Gene | Name | Accession number1 | Forward primer (5′-3′) | Reverse primer (5′-3′) | Annealing temperature (°C) | Amplicon size (bp) | Efficiency of primers pairs (%) |
| 60RSP | 60S ribosomal protein L18 | XM_002270599 1 | ATCTACCTCAAGCTCCTAGTC | CAATCTTGTCCTCCTTTCCT | 60 | 166 | 100.0 |
| EF1 | Elongation factor 1-alpha | XM_002284888 1 | AACCAAAATATCCGGAGTAA AAGA | GAACTGGGTGCTTGATAGGC | 60 | 164 | 100.0 |
| LOX9 | Lipoxygenase | NM_001281249 1 | CCCTTCTTGGCATCTCCCTTA | TGTTGTGTCCAGGGTCCATTC | 60 | 101 | 90.0 |
| PR1 | Pathogenesis- related protein 1 | XM_002273752 1 | GGAGTCCATTAGCACTCCTTTG | CATAATTCTGGGCGTAGGCAG | 60 | 168 | 90.0 |
| PR2 | Class I beta-1,3-glucanase | NM_0012809671 | TCAATGGCTGCAATGGTGC | CGGTCGATGTTGCGAGATTTA | 60 | 155 | 97.2 |
| GST1 | Glutathione-S-transferase | NM_001281248 1 | TGCATGGAGGAGGAGTTCGT | CAAGGCTATATCCCCATTTTCTTC | 60 | 98 | 90.0 |
| PAL | Phenylalanine ammonia lyase | XM_003635637 1 | TCCTCCCGGAAAACAGCTG | TCCTCCAAATGCCTCAAATCA | 60 | 101 | 92.9 |
| STS | Stilbene synthase | NM_001281117 1 | AGGAAGCAGCATTGAAGGCTC | TGCACCAGGCATTTCTACACC | 60 | 101 | 94.3 |
1NCBI accession number.