TABLE 2.
Primer sequence used for PCR, ddPCR, and NGS in this study.
| Platforms | Primer name | Oligonucleotide sequence (5′–3′) | Purpose |
| QX 200 | 337F | GACTCCTACGGGAGGCWGCAG | ddPCR quantification |
| 518R | CGTATTACCGCGGCTGCTGG | ||
| MiSeq | MiSeq_27F | TCGTCGGCAGCGTC-AGATGTGTATAAGAGACAG-AGAGTTTGATCMTGGCTCAG | Genomic DNA amplicon |
| MiSeq_bif27F | TCGTCGGCAGCGTC-AGATGTGTATAAGAGACAG-GGGTTCGATTCTGGCTCAG | ||
| MiSeq_337R | GTCTCGTGGGCTCGG-AGATGTGTATAAGAGACAG-CTGCWGCCTCCCGTAGGAGTC | ||
| MiSeq_337F | TCGTCGGCAGCGTC-AGATGTGTATAAGAGACAG-GACTCCTACGGGAGGCWGCAG | ||
| MiSeq_518R | GTCTCGTGGGCTCGG-AGATGTGTATAAGAGACAG-CGTATTACCGCGGCTGCTGG | ||
| MiSeq_518F | TCGTCGGCAGCGTC-AGATGTGTATAAGAGACAG-CCAGCAGCCGCGGTAATACG | ||
| MiSeq_800R | GTCTCGTGGGCTCGG-AGATGTGTATAAGAGACAG-TACCAGGGTATCTAATCC | ||
| I5 forward primer | AATGATACGGCGACCACCGAGATCTACAC-XXXXXXXX-TCGTCGGCAGCGTC | Nextera barcoding | |
| I7 reverse primer | CAAGCAGAAGACGGCATACGAGAT-XXXXXXXX-GTCTCGTGGGCTCGG | ||
| IonTorrent | 27F | AGAGTTTGATCMTGGCTCAG | Genomic DNA amplicon |
| bif27F | GGGTTCGATTCTGGCTCAG | ||
| 337R | CTGCWGCCTCCCGTAGGAGTC | ||
| 337F | GACTCCTACGGGAGGCWGCAG | ||
| 518R | CGTATTACCGCGGCTGCTGG | ||
| 518F | CCAGCAGCCGCGGTAATACG | ||
| 800R | TACCAGGGTATCTAATCC | ||
| MGIseq-2000 | 337F | GACTCCTACGGGAGGCWGCAG | |
| 518R | CGTATTACCGCGGCTGCTGG | ||
| Sequel II MinION | 27F | AGAGTTTGATCMTGGCTCAG | |
| bif27F | GGGTTCGATTCTGGCTCAG | ||
| 1492R | TACGGYTACCTTGTTACGACTT |