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. 1999 Oct;37(10):3175–3178. doi: 10.1128/jcm.37.10.3175-3178.1999

FIG. 1.

FIG. 1

PCR amplification of the ITS for M. mycetomatis and related species. Lanes 1 to 8 show the amplicons obtained by using the universal primers ITS4 and ITS5. DNAs from the following species were amplified: M. grisea CBS331-50 (lane 1) and CBS 332-50 (lane 2), M. mycetomatis CBS 247.48 (lane 3) and CBS 868.95 (lane 4), P. mackinnonii CBS 674.75 (lane 5), P. romeri CBS 252.60 (lane 6), P. unguis-homini CBS 378.92 (lane 7), and C. larense CBS 640.73 (lane 8). In lanes 9 to 16 PCR results obtained with the primer combination 26.1A and 28.3A are displayed; the strains are ordered similarly from left to right. Note that only M. mycetomatis CBS 247.48 yielded a positive signal. Lanes 17 to 19 show the representative PCR products obtained for all of the clinical M. mycetomatis isolates. Lane 20 contains the negative PCR control. On the left and right the molecular size of the intensely fluorescing 600-bp-long fragment in the 100-bp ladder is indicated.