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. 1999 Oct;37(10):3175–3178. doi: 10.1128/jcm.37.10.3175-3178.1999

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Copyright © 1999, American Society for Microbiology

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FIG. 2 — RFLP analysis of the ITS amplicons obtained for M. mycetomatis and related species. Lanes 1 to 8 show the results obtained for M. grisea CBS331-50 (lane 1) and CBS 332-50 (lane 2), M. mycetomatis CBS 247.48 (lane 3) and CBS 868.95 (lane 4), P. mackinnonii CBS 674.75 (lane 5), P. romeri CBS 252.60 (lane 6), P. unguis-homini CBS 378.92 (lane 7), and C. larense CBS 640.73 (lane 8). The three panels were generated with the restriction enzymes indicated. Lanes 9 to 16 show the results obtained for eight of the clinical M. mycetomatis isolates, indicating the high degree of ITS homogeneity. Lanes M contain the 100-bp size marker ladder; the position of the 600-bp-long fragment is indicated.