Figure 5.

Bi-specific chimeric antigen receptor (CAR) T cells show different in vivo antitumor efficacy. Nalm6-bearing NSG mice were treated with 1x10⁶ CAR T cells 7 days after initial tumor cell injection. (A) Bioluminescent images of each mouse per condition at specified days after T/CAR T cell injection. (B) Tumor burden (average radiance) of each mouse treated with bi-specific CAR T cells and controls. Two-way analysis of variance (ANOVA) was performed with Dunnet’s multiple comparison test against untransduced T cell (UT). Each line represents an individual mouse. (C) Kaplan-Meier analysis of event-free survival defined as time in days to an average luminescence equal or greater than 1x10⁶ p/s/cm²/sr of mice treated with 1x10⁶ T/CAR T cells compared with UT control (n = 8 mice per group). *p<0.05.