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. 2021 Oct 26;218(12):e20211872. doi: 10.1084/jem.20211872

Figure 5.

Figure 5.

IL-20 regulates Dnmt3a-mediated osteoclastogenesis. (A) Effect of increasing concentrations of IL-20 on osteoclast differentiation from 200 CD11b−/lowCD115+Ly6Chi osteoclast precursors sorted from the BM of 12-wk-old Dnmt3a−/− mice, shown as boxplots. n = 11. (B) Co-culture experiments of 200 CD11b−/lowCD115+Ly6Chi osteoclast precursors from WT or Dnmt3a−/− mice and 2,000 BMDMs from respective sources in the presence of IL-20 neutralizing antibody 7E or isotype IgG during osteoclast differentiation. Boxplot representation of TRAP+ cells at day 6 of differentiation. n = 8. (C) Expression of the Ctsk reporter (APC+) as a percentage of GFP+ cells using Ctsk-T2A-R647 reporter cell line expressing Cas9-GFP in the presence WT or Dnmt3a−/−, and IL-20 neutralizing antibody 7E or isotype antibody during osteoclast differentiation. n = 8. (D) WT or Dnmt3a−/− BM transplants into WT male mice treated with isotype antibody or IL-20 neutralizing antibody 7E at a dose of 6 μg/kg every 3 d intraperitoneally starting at 8 wk after transplantation for 4 wk. Serum was collected at 12 wk and analyzed for CTX-I, a marker of bone resorption. Statistical significance via Wilcoxon rank sum test and adjusted by FDR. n = 7–14. For A–C, one-way ANOVA was used, and P values were adjusted for multiple comparisons using BY. *, P < 0.05; **, P < 0.01. Error bars otherwise note SD.