TABLE 1.
MAb | G serotype reactivity in decreasing order | Immunoglobulin classa | Reciprocal FFN titer with 116E | Fold change in FFN titer to 116E over other that to G9 virusesb | Reciprocal direct EIA titer with 116E | Fold change in EIA titer to 116E over other that to G9 virusesb |
---|---|---|---|---|---|---|
F45:6 | 9, 5 | M | 1 × 103 | <−1,000 to −200 | 1 × 103 | −300 to −30 |
F45:5 | 9 | G3 | 5 × 103 | −16 to −6.0 | 3 × 104 | −66 to −3.0 |
WI61:1 | 9, 8, 5 | M | 5 × 104 | <−20 to −4.0 | 6 × 104 | −18 to 1.0 |
F45:7 | 9, 5 | M | 2 × 103 | −150 to −50 | 2 × 103 | −30 to −15 |
F45:9 | 9 | M | 1 × 105 | −10 to −0.5 | 4 × 103 | −100 to −75 |
F45:8 | 9 | M | <1 × 102 | <−104 to <−100 | 8 × 102 | −5,000 to −125 |
RV-3:2 | 3, 9, 5 | G2bc | 3 × 102 | −170 to −67 | 3 × 103 | −660 to 1.5 |
RV-3:4 | 3, 9 | G1 | 4 × 102 | −50 to >4.0 | 2 × 104 | 1.0 to 10 |
F45:1 | 9, 4 | G2a | >1 × 106 | 1.0 to >5.0 | >1 × 106 | ≥−4.0 to >1.0 |
F45:2 | 9, 3, 4 | A | 4 × 103 | −2.5 to 4.0 | 8 × 103 | −125 to −4.0 |
Determined with an EIA kit with subclass- and isotype-specific antisera (Commonwealth Serum Laboratories, Parkville, Victoria, Australia).
The other G9 rotaviruses tested were F45, WI61, and AU32. FFN and EIA titers of MAbs F45:1, F45:2, F45:5, F45:6, F45:7, F45:8, F45:9, and WI61:1 with these rotaviruses were determined previously (34).
Described previously (7).