TABLE 2.

Reactivities of MAbs used to distinguish G types 1 to 4 and 9 by serotyping EIA with a panel of cell culture-adapted rotaviruses

Rotavirus strain	G serotype	Reciprocal titer of MAb F45:1 in serotyping EIA formata	OD450 with given MAb (MAb G serotype specificity)b:
			F45:1 (9)	RV-4:2 (1)	RV-5:3 (2)	RV-3:1 (3)	ST-3:1 (4)	60 (VP7)
116E	9	>1 × 107	1.283	0.184	0.136	0.173	0.116	1.450
F45	9	>1 × 107	2.768	0.200	0.153	0.199	0.243	2.657
WI61	9	>1 × 107	0.970	0.179	0.127	0.172	0.215	1.462
AU32	9	>1 × 107	0.463	0.187	0.124	0.168	0.210	0.747
Hoso	4	2 × 106	2.586	0.040	0.039	0.040	2.495	2.868
ST-3	4	2 × 104	0.130	0.038	0.036	0.043	1.654	2.839
RV-4	1	5 × 103	0.127	2.491	0.037	0.039	0.039	2.791
RV-5	2	7 × 103	0.133	0.040	0.362	0.035	0.036	0.955
SA11	3	5 × 103	0.110	0.041	0.038	2.680	0.045	2.977
TFR41	5	5 × 103	0.104	0.038	0.037	0.041	0.220	0.879

^aAll rotavirus strains tested reacted with MAb F45:1. However, the dilution of MAb F45:1 used to screen samples in the serotyping EIA was higher than its titers with RV-4, RV-5, SA11, and TFR41, so these rotaviruses were not detected in the serotyping EIA with the single optimal dilution of MAb F45:1. 

^bPositive reactions in the serotyping EIA, in which MAbs were used at a single optimal dilution, are shown in boldface.