TABLE 3.
Comparison of MAb serotyping EIA and RT-PCR for determination of G9 types of cell culture-adapted human rotaviruses
| G genotype determined by RT-PCR | No. of strains with the following G serotype determined by MAb EIAa:
|
Total | ||||
|---|---|---|---|---|---|---|
| 9 | 9+3 | 9+4 | 3 | Nontypeable | ||
| 9 | 13 | 1 | 1 | 0 | 3 | 18 |
| 9+3 | 1 | 0 | 0 | 0 | 0 | 1 |
| 2 | 0 | 0 | 0 | 0 | 2 | 2 |
| 3 | 0 | 0 | 0 | 1 | 0 | 1 |
| Nonreactiveb | 2 | 0 | 0 | 0 | 0 | 2 |
| Total | 16 | 1 | 1 | 1 | 5 | 24 |
a
All samples that contained viral antigen typeable by EIA also reacted with MAb 60, whereas none of the samples that contained nontypeable antigen reacted with MAb 60.
b
No RT-PCR product was visible, even though a product of the expected size from control prototype rotaviruses was obtained.