TABLE 4.
Comparison of MAb serotyping EIA and RT-PCR for determination of G9 type in stool extracts containing human rotaviruses
| G genotype determined by RT-PCR | No. of strains with the following G serotype determined by MAb EIA:
|
Total | |||
|---|---|---|---|---|---|
| 9 | 9+4 | 3 | Nontypeablea | ||
| 9 | 23 | 1 | 0 | 16 | 40 |
| 9+1 | 0 | 0 | 0 | 1 | 1 |
| 9+2 | 1 | 0 | 0 | 0 | 1 |
| 3 | 0 | 0 | 1 | 0 | 1 |
| Nonreactiveb | 0 | 0 | 0 | 4 | 4 |
| Not tested | 1 | 0 | 0 | 2 | 3 |
| Total | 25 | 1 | 1 | 23 | 50 |
a
Only 12 (63%) of the stools containing rotavirus typeable or not tested by RT-PCR and not typeable by EIA (n = 19) contained VP7 antigen detectable with MAb 60, whereas all stools that contained viral antigen typeable by EIA also reacted with MAb 60.
b
See footnote b of Table 3.