Table 1b. Intraday and Interday Precision Test Result by HPLC-DAD and GC-FIDa.
| DNPH method HPLC-DAD |
p-toluenesulfonic acid liquid GC-FID |
|||||
|---|---|---|---|---|---|---|
| parameter | day I | day II | day III | day I | day II | day III |
| average (%) | 101.28 | 101.80 | 101.90 | 101.37 | 100.18 | 100.08 |
| SD | 0.38 | 0.43 | 0.27 | 1.26 | 1.07 | 1.34 |
| RSD (%) | 0.37 | 0.42 | 0.27 | 1.24 | 1.06 | 1.34 |
| Horwitz (%) | 5.32 | 5.32 | 5.32 | 5.32 | 5.33 | 5.33 |
| Horrat | 0.07 | 0.08 | 0.05 | 0.23 | 0.20 | 0.25 |
| RSD (%) combined | 0.44 | 1.15 | ||||
| confidence Interval (%) | 100.98–102.14 | 98.59–102.61 | ||||
a
DNPH (2,4-dinitrophenylhydrazine). HPLC-DAD (high-performance liquid chromatography-diode array detector). SHS-GC-MS (static headspace-gas chromatography-mass spectrometry). GC-FID (gas chromatography-flame ionization detection). RSD (relative standard deviation), Horwitz and Horrat: acceptance criteria of the accuracy and precision test (AOAC guidelines).