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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Neuropharmacology. 2021 Jul 9;197:108698. doi: 10.1016/j.neuropharm.2021.108698

Figure 1. Timeline of operant self-administration and brain collection followed by gene expression and immunohistochemical analyses.

Figure 1.

Rats were weaned at PD21 and started operant training at PD25, followed by self-administration of sweetened alcohol (alcohol groups) or water (control groups) from PD28–42. Brains were collected at PD43 and processed for immunohistochemical analysis of microglia cells (Experiment 1) or qPCR analysis of neuroimmune-related gene expression (Experiment 2). Created with BioRender.com