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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Neuropharmacology. 2021 Jul 9;197:108698. doi: 10.1016/j.neuropharm.2021.108698

Figure 4. Alcohol drinking produces a shift in microglial cell morphology to a more reactive state in the hippocampus of adolescent males and females.

Figure 4.

A) 3 cropped images were taken for each of the following regions for analysis: DG, CA1, CA2/3, shown by purple, blue, and green squares, respectively. B) Automated morphological analysis of microglial cells was conducted using Nikon NIS-Elements Advanced Research Software. C) The alcohol group in males and females for the CA1, CA2/3 and in females for the DG shifted the population of microglial cell morphology to the left, indicating a state of greater activation, characterized by retracted branchings. The DG of the male alcohol group had a shift to the right, indicating greater branching points, which supports an intermediate “hyper-ramified” activation state (n=3–6 rats for each sex and treatment group. Two-sample Kolmogorov-Smirnov test, p < 0.05).