Figure 3.

Accurate analysis of HLA ligands isolated from the JY cell line. (A) The eluted 9mers were analyzed in regards to their binding affinity to HLA-A*02:01 and HLA-B*07:02. The binders (green dots) and nonbinders (black dots) were defined in the NetMHCpan 4.0 Server (applied rank 2%). (B) HLA-I consensus binding motifs. Gibbs clustering analysis was performed to define the consensus binding motifs among the eluted 9mers peptides. The reference motif is depicted in the upper right corner. The clusters with the optimal fitness (higher KLD values, orange star) are shown, and the sequence logo is represented with the number of HLA-I for each cluster.