TABLE 2.
Number of reference strains exhibiting the indicated RFLP patterns obtained from restriction digests of the amplified variable region of the 16S rRNA gene
| Patternb | No. of strains in speciesa
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ah | Ab | Asa | Ac | Am | Aeu | Aso | Avs | Aj | Avv | Aen | Asc | At | Aa | Totalc | |
| A/a | 5 | 1 | 6 | ||||||||||||
| A/b | 4 | 6 | 10 | ||||||||||||
| A/c | 4 | 4 | |||||||||||||
| B/c | 1 | 1 | |||||||||||||
| B/d | 6 | 3 | 2 | 11 | |||||||||||
| B/e | 3 | 3 | |||||||||||||
| C | 5 | 1 | 3 | 9 | |||||||||||
| D | 1 | 3 | 4 | ||||||||||||
| E/a | 1 | 1 | |||||||||||||
| E/c | 2 | 2 | |||||||||||||
| F | 4 | 3 | 1 | 8 | |||||||||||
| G | 3 | 3 | |||||||||||||
Ah, A. hydrophila; Ab, A. bestiarum; Asa, A. salmonicida; Ac, A. caviae; Am, A. media; Aeu, A. eucrenophila; Aso, A. sobria; Avs, A. veronii biovar sobria; Aj, A. jandaei; Avv, A. veronii biovar veronii; Aen, A. encheleia; Asc, A. schubertii; At, A. trota; Aa, A. allosaccharophila.
RFLP pattern. Capital letters refer to the pattern resulting from AluI digests; lowercase letters a, b, and c refer to CfoI digests; and d and e refer to MnlI digests. A strain with pattern combination A/a had pattern A when the PCR product was digested with AluI and pattern a when digested with CfoI.
Sum of the strains with a particular pattern.