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. Author manuscript; available in PMC: 2022 Oct 16.
Published in final edited form as: Cell Physiol Biochem. 2021 Oct 16;55(5):605–617. doi: 10.33594/000000438

Fig. 4-.

Fig. 4-

Immunofluorescence analysis of histone-mediated VE-cadherin disruption in endothelial cells. (A) Primary HUVECs were incubated with Histone H3 (1 µM) for 1h in the absence or presence of WT-AT and AT-4Mut (2.5 µM each). Cells were then fixed, permeabilized and incubated with rabbit anti-VE-cadherin antibody and Alexa Fluor 488-conjugated goat anti-rabbit IgG. The nucleus was stained with DAPI. Immunofluorescence images were obtained with confocal microscopy. (B) The same as (A) except that primary HUVECs were incubated with histone H3 for 1h in the absence or presence of surfen (10 µM). Arrows indicate loss of VE-cadherin at junctions.