TABLE 2.
Clinical M. tuberculosis strains identified to carry an IS6110 insertion element in genes whose disruption confers antibiotic resistancea
| M. tuberculosis genome | Gene with IS6110 insertion | Associated resistance to clinical antibiotic | Insertion position/gene length (bp) | Confirmation by read-based analysis | Geographic location |
|---|---|---|---|---|---|
| TB_RSA126 | Rv0678 | Bedaquiline/clofazimine | 71/498b | Yes | South Africa |
| TB_RSA64 | Rv0678 | Bedaquiline/clofazimine | 71/498b | Yes | South Africa |
| TB_RSA63 | Rv0678 | Bedaquiline/clofazimine | 71/498b | Yes | South Africa |
| TKK-01-0074 | Rv0678 | Bedaquiline/clofazimine | 76/498 | Yes | South Africa |
| KT-0084 | Rv0678 | Bedaquiline/clofazimine | 71/498 | Yes | South Korea |
| Strain 02-R0861 | ethA | Ethionamide | 1,267/1,470c | Yes | Lima, Peru |
| Strain 99-09115 | ethA | Ethionamide | 1,267/1,470c | Reads not available | Lima, Peru |
| Strain 02-R0014 | pncA | Pyrazinamide | 159/561c | Reads not available | Lima, Peru |
| Strain 01-R0774 | pncA | Pyrazinamide | 159/561c | Yes | Lima, Peru |
| XTB13-251 | pncA | Pyrazinamide | 462/561 | Yes | Belarus |
| KT-0109 | thyA | PAS | 84/792 | Yes | South Korea |
| KT-0077 | thyA | PAS | 150/792 | Yes | South Korea |
a
Mapped reads can be visualized in Fig. S2 in the supplemental material. PAS, para-aminosalicylic acid.
b
Identical by IS6110 barcode: different clinical isolate, same M. tuberculosis strain.
c
Identical by contig-based genotype comparison: different clinical isolate, same M. tuberculosis strain.