FIG. 4.

DCP-1 cleaves full-length DCP-1 and full-length drICE, but drICE does not cleave full-length drICE. ³⁵S-labeled full-length DCP-1 and full-length drICE were incubated with the purified active form of DCP-1 or drICE at 37°C for 30 min. After incubation the proteins were separated by SDS–10% PAGE under reducing conditions. The gels were dried and exposed to X-ray films for visualization. (A) ³⁵S-labeled full-length DCP-1 was treated with active DCP-1 or active drICE; (B) ³⁵S-labeled full-length drICE was treated with active DCP-1 or active drICE.