TABLE 2.
Prospective comparison of whole genome sequencing with the VOC assaysa
| VOC assay result | Whole genome sequencing result |
% Sensitivity (95% CI) | % Specificity (95% CI) | % PPV (95% CI) | % NPV (95% CI) | |
|---|---|---|---|---|---|---|
| Positive | Negative | |||||
| B.1.1.7 | ||||||
| Positive | 557 | 0 | 98.2 (96.8–99.2) | 100 (99.9–100) | 100 | 99.7 (99.4–99.9) |
| Negative | 10 | 2,984 | ||||
| P.1/B.1.351 | ||||||
| Positive | 123 | 37 | 100 (97.1–100) | 98.9 (98.5–99.2) | 76.9 (70.7–82.1) | 100 |
| Negative | 0 | 3,391 | ||||
| Wild-type | ||||||
| Positive | 2,832 | 0 | 99 (98.6–99.3) | 100 (99.5–100) | 100 | 96 (94.3–97.2) |
| Negative | 29 | 690 | ||||
a
VOC assay result was defined as positive for B.1.1.7 if both ΔH69/V70 and N501Y assays were positive, positive for P.1/B.1.351 if only the N501Y assay was positive and the ΔH69/V70 assay was negative, and positive for wild-type if both ΔH69/V70 and N501Y assays were negative. PPV, positive predictive value; NPV, negative predictive value; CI, confidence interval.