TABLE 5.

Primers and probes for the detection of the H69/V70 deletion and N501Y mutations in variants of concern for SARS-CoV-2

Target	Primer/probe name	Primer/probe sequence (5′–3′)
H69/V70 deletion	Covid_Spk69/70_For	AGTTTTACATTCAACTCAGGACTTGTTC
	Covid_Spk69/70_Rev	GACAGGGTTATCAAACCTCTTAGTACC
	Covid-Spk6970_WT	CATGCTATACATGTCTCTGG_FAM_MGB
	Covid-Spk6970_D	CATGCTATCTCTGGGACC_VIC_MGB
N501Y	Covid_SpkN501Y_For	ACACCTTGTAATGGTGTTGAAGG
	Covid_SpkN501Y_Rev	AGTTGCTGGTGCATGTAGAAGTTC
	Covid-SpkN501Y_WT	CAACCCACTAATGGTGTTGG_FAM_MGB
	Covid-SpkN501Y_M	CAACCCACTTATGGTGTTGG_VIC_MGB