Fig. 1.

Schematic representations of the Spike protein and the 1006 bp amplicon. PCR amplifications were performed with primers that were used both for PCR and Sanger sequencing (blue arrows). Yellow arrows indicate primers used only for sequencing. Colored rectangles represent changes in amino acids found in different VOI and VOC's and their respective positions in the SARS-CoV-2 genome, Lysine (K) blue, Glutamic acid (E) orange, Asparagine (N) green, Alanine (A) purple, Aspartic acid (D) yellow, Valine (V) dark green. It is also represented the 969 bp PCR amplificon generated with primers annealing within the spike and orf3a protein genes (small blue arrows), which allowed us to verify for the presence or absence of a mutation at position 1176 that differs in the VOIs N.9 and P.2. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)