KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
NeuN (Rabbit)	Abcam	Cat# Ab177487; RRID:AB_2532109
GFP (Chicken)	Aves	Cat# GFP-1020; RRID:AB_10000240
FOXP2 (Rabbit)	Abcam	Cat# Ab16046; RRID:AB_2107107
Estrogen receptor 1 (Rabbit)	Millipore	Cat# 06–935; RRID:AB_310305
GFAP (Chicken)	Abcam	Cat# ab4674; RRID:AB_304558
Iba1 (Rabbit)	Fuji Film	Cat# 019–19741; RRID:AB_839504
S100beta (Rabbit)	Abcam	Cat# ab52642; RRID:AB_882426
ARC (Rabbit)	Synaptic Systems	Cat# 156003; RRID:AB_887694
c-Fos (Mouse)	Santa Cruz Biotechnology	Cat# Sc-166940; RRID:AB_10609634
Alexa Fluor 647 AffiniPure Fab Fragment Donkey Anti-Rabbit IgG (H+L)	Jackson Immune	Cat# 711–607-003; RRID:AB_2340626
Goat Anti-Chicken IgY H&L (Alexa Fluor 555)	Abcam	Cat# ab150170
Alexa Fluor 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Chicken IgY (IgG) (H+L)	Jackson Immune	Cat# 703–546-155; RRID:AB_2340376
Donkey Anti-Mouse IgG H&L (Alexa Fluor 555)	Abcam	Cat# Ab150106; RRID:AB_2857373
HCR probes	IDT
Bacterial and virus strains
AAV-DJ EF1a-DIO-GCaMP6f	Stanford	GVVC-AAV-93
AAV5.Syn.Flex.GCaMP6s.WPRE.SV40	Addgene	100845-AAV5
pGP-AAV-syn-jGCaMP7b-WPRE	Addgene	104489-AAV1
pAAV.Syn.GCaMP6s.WPRE.SV40	Addgene	100843-AAV9
pAAV-FLEX-tdTomato	Addgene	28306-AAV9
Chemicals, peptides, and recombinant proteins
Cocaine	Sigma-Aldrich	C5776–1G
Normal Donkey Serum	Jackson Immune	017–000-121
Dimethyl sulfoxide	Fisher	D128–4
Heparin	Sigma-aldrich	H3393–100KU
Triton X-100	Sigma-aldrich	93443–100ML
TWEEN 20	Sigma-aldrich	3005
Glycine	Sigma-aldrich	G7126–100G
D-Fructose	Sigma-aldrich	F0127–1KG
1-Thioglycerol	Sigma-aldrich	M1753–100ML
Formamide	Millipore	344206–100ML
SSC	Thermofisher	AM9763
Tween-20	Sigma-aldrich	P9416
Ribonucleoside vanadyl complex	BioLabs	S1402S
Salmon sperm DNA	Thermo-Fisher	15632011
hairpins	Molecular Technologies	N/A
Experimental models: Organisms/strains
VIP-cre X Ai162 (GC6s)	Jackson Laboratory Stock	010908, 031562
VIP-cre X Ai140 (GFP)	Jackson Laboratory Stock	010908, 30220
DAT-cre	Jackson Laboratory Stock	6660
TH-cre	EMMA, Ted Ebendal	254
C57BL/6N	Charles River	C57BL/6NCrl
Drd1-cre	Jackson Laboratory Stock	030989-UCD
Drd2-cre	Jackson Laboratory Stock	032108-UCD
Thy1-YFP	Jackson Laboratory Stock	3782
Software and algorithms
ImageJ, version 2.0.0-rc-69/1.52n	NIH, open source	https://fiji.sc/
Adobe Illustrator, 24.0.2 (64-bit)	Adobe	https://www.adobe.com/products/illustrator.html
MATLAB R2018a, R2020b	MathWorks Inc.	https://www.mathworks.com/products/matlab.html
ABET II, Model 89501	Lafayette Instrument Company	https://lafayetteneuroscience.com/
Imaris 9.2.0	Oxford Instruments	https://imaris.oxinst.com/
Zen (LSM 880 with and without Airyscan, 2.3 lite)	Zeiss Microscopy	https://www.zeiss.com/corporate/int/home.html
BZ-X700 microscope and BZ-X Analyzer	Keyence	https://www.keyence.com
BioRender	BioRender	https://biorender.com/
LabVIEW 2016 (32bit)	National Instruments	https://www.ni.com/en-us.html
Custom code to analyze and generate figures	This paper	https://zenodo.org/record/5329620
Other
Optical fiber, 400 μm diameter, 7 mm long	Doric Lenses	MFC_400/430–0.48_7mm_ZF1.25_FLT
Optical fiber, 400 μm diameter, 3 mm long (For distance visualization)	Doric Lenses	MFC_400/430–0.48_3mm_ZF1.25_FLT
Optical fiber, 200 μm diameter, 4 mm long (For distance visualization)	Doric Lenses	MFC_200/240–0.22_4mm_ZF1.25_FLT
Mono Fiberoptic Patch cable	Doric Lenses	MFC_400/430_0.48_2m_FC_ZF1.25_FL
GRIN lens, 500 μm diameter, 8.4 mm long	Inscopix	GLP-0584,
GRIN lens, 600 μm diameter, 7.3 mm long	Inscopix	GLP-0673
GRIN lens, 500 μm diameter, 8.85 mm long	GRIN-tech	NEM-050–30-10–920-S-2.0p
Custom ferrule for holding GRIN lens	Kientec Systems	FZI-LC-L2.5–520